Product details

Background:
CD8 is a 68 kDa transmembrane glycoprotein consists of two subunits, αβ heterodimer or αα homodimer linked by a disulfide. CD8 is mainly expressed on the surface of suppress/cytotoxic T cells and NK cells. It is detected to be positive in T-cell large granular lymphocytic leukemia and subcutaneous panniculitis like T-cell lymphoma, and can be a marker for Precursor T lymphoblastic leukemia / lymphoma. CD8 is negative in Mycosis Fungoides and non-hematolymphoid neoplasms.

Specifications

Size	50μL/100μL
Target	CD8
Reactivity	Human
Applications	IHC, WB, IF, ELISA
MW(Calculated)	26kD
MW(Observed)	30-35kD
Host Species	Mouse
Isotype	IgG2b,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMM304
Immunogen	Synthesized peptide derived from human protein. AA range:100-235
Specificity	The antibody can specifically recognize human CD8 protein, including two typies of dimer: αβ heterodimer or αα homodimer.
Gene Name	CD8A MAL
Protein Name	CD8 a
Other Name	alpha polypeptide; / p32; / CD_antigen=CD8a; / CD8; / CD8 antigen alpha polypeptide; / CD8 antigen alpha polypeptide; / p32; / CD8 antigen, alpha polypeptide; / p32; / CD8a; / CD8A antigen; / CD8A molecule; / CD8A_HUMAN; / Leu2; / Leu2 T lymphocyte antigen; / Ly 2; / Ly 35; / Ly B; / Ly2; / Ly3; / Ly35; / LyB; / Lyt 2.1 lymphocyte differentiation antigen; / AA at 100; / LYT3; / MAL; / OKT8 T cell antigen; / OTTHUMP00000160760; / OTTHUMP00000160764; / OTTHUMP00000203528; / OTTHUMP00000203721; / p32; / T cell antigen Leu2; / T cell co receptor; / T lymphocyte differentiation antigen T8/Leu 2; / T-cell surface glycoprotein CD8 alpha chain; / T-cell surface glycoprotein Lyt 2; / T-lymphocyte differentiation antigen T8/Leu-2; / T8 T cell antigen; / T8/Leu-2 T-lymphocyte differentiation antigen
Species	Human
Gene ID	925
Uniprot	P01732
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Membranous
Tissue Expression	CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. Less frequently, CD8 can be expressed as a CD8A homodimer. A subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes and dendritic cells expresses CD8A homodimers. Expressed at the cell surface of plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.
Signaling_pathway	Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Human Diseases >> Immune disease >> Primary immunodeficiency;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Cell adhesion molecules; / >>Antigen processing and presentation; / >>Hematopoietic cell lineage; / >>T cell receptor signaling pathway; / >>Yersinia infection; / >>Primary immunodeficiency
Function	Disease:Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.,Function:Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.,online information:CD8 entry,online information:CD8A mutation db,PTM:All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.,

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CD8 a [ABT304] mouse mAb

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CD8 a [ABT304] mouse mAb

Product details

Specifications

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