Product details

Background:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011],

Specifications

Size	50μL/100μL
Target	CD8
Reactivity	Human
Applications	IHC, IF, ELISA
MW(Calculated)	26kD
MW(Observed)	30-35kD
Host Species	Mouse
Isotype	IgG2b,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-400; IF 1:200-1:1000; ELISA 1:500-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMM304
Immunogen	Synthesized peptide derived from human CD8 AA range: 100-235
Specificity	The antibody can specifically recognize human CD8 protein, including two typies of dimer: αβ heterodimer or αα homodimer.
Gene Name	CD8A MAL
Protein Name	alpha polypeptide (p32); CD_antigen=CD8a; CD8; CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide (p32); CD8 antigen, alpha polypeptide (p32); CD8a; CD8A antigen; CD8A molecule; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; Ly 2; Ly 35; Ly B; Ly2; Ly3; Ly35; LyB; Lyt 2.1 lymphocyte differentiation antigen (AA at 100); LYT3; MAL; OKT8 T cell antigen; OTTHUMP00000160760; OTTHUMP00000160764; OTTHUMP00000203528; OTTHUMP00000203721; p32; T cell antigen Leu2; T cell co receptor; T lymphocyte differentiation antigen T8/Leu 2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt 2; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen; T8/Leu-2 T-lymphocyte differentiation antigen
Other Name	alpha polypeptide; / p32; / CD_antigen=CD8a; / CD8; / CD8 antigen alpha polypeptide; / CD8 antigen alpha polypeptide; / p32; / CD8 antigen, alpha polypeptide; / p32; / CD8a; / CD8A antigen; / CD8A molecule; / CD8A_HUMAN; / Leu2; / Leu2 T lymphocyte antigen; / Ly 2; / Ly 35; / Ly B; / Ly2; / Ly3; / Ly35; / LyB; / Lyt 2.1 lymphocyte differentiation antigen; / AA at 100; / LYT3; / MAL; / OKT8 T cell antigen; / OTTHUMP00000160760; / OTTHUMP00000160764; / OTTHUMP00000203528; / OTTHUMP00000203721; / p32; / T cell antigen Leu2; / T cell co receptor; / T lymphocyte differentiation antigen T8/Leu 2; / T-cell surface glycoprotein CD8 alpha chain; / T-cell surface glycoprotein Lyt 2; / T-lymphocyte differentiation antigen T8/Leu-2; / T8 T cell antigen; / T8/Leu-2 T-lymphocyte differentiation antigen
Species	Human
Gene ID	925
Uniprot	P01732
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Membranous
Tissue Expression	Tonsil/ Appendix
Signaling_pathway	Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Human Diseases >> Immune disease >> Primary immunodeficiency;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Cell adhesion molecules; / >>Antigen processing and presentation; / >>Hematopoietic cell lineage; / >>T cell receptor signaling pathway; / >>Yersinia infection; / >>Primary immunodeficiency
Function	Disease:Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.,Function:Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.,online information:CD8 entry,online information:CD8A mutation db,PTM:All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterdimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.,

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CD8 a [ABT304] mouse mAb

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CD8 a [ABT304] mouse mAb

Product details

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