Product details

Background:
Cytokeratin 5/6 are high molecular weight keratins, which are normally expressed in squamous epithelium, basal cells of ductal epithelium, mesothelial cells, myoepithelium and basal cells of prostate, but not in glandular epithelium. Most adenocarcinoma are negative, and some adenocarcinoma can be weakly positive. The antibody is mainly used for the differential diagnosis of mesothelioma and adenocarcinoma, as well as the study of benign and malignant tumors of epithelial origin.

Specifications

Size	50μL/100μL
Target	Cytokeratin 5/6
Reactivity	Human
Applications	IHC, WB, IF, ELISA
MW(Calculated)
MW(Observed)	60,62kD
Host Species	Mouse
Isotype	IgG2a,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMM-CK5/052
Immunogen	Synthesized peptide derived from human Cytokeratin 5 and Cytokeratin 6 AA range: C terminal amino acids 500-590 of Cytokeratin 5 and N terminal amino acids 2-100 of Cytokeratin 6
Specificity	The antibody can recognize both human CK5 and CK6 protein, and shows no cross reaction with other typies of cytokeratin. In western blotting of A431 cell lysate, there shows a 56 kDa band dectected by the antibody.
Gene Name	KRT5 KRT6A KRT6B KRT6C
Protein Name	Keratin； type II cytoskeletal 5 ；58 kDa cytokeratin；Cytokeratin-5；CK-5；Keratin-5；K5；Type-II keratin Kb5；type II cytoskeletal 6A; Cytokeratin-6A; CK-6A; Keratin-6A; K6A; Type-II keratin Kb6; allergen Hom s 5; type II cytoskeletal 6B; Cytokeratin-6B; CK-6B; Keratin-6B; K6B; type II cytoskeletal 6C; Cytokeratin-6C; CK-6C; Keratin-6C; K6C
Other Name	58 kDa cytokeratin; / CK-5; / CK5; / Cytokeratin-5; / Cytokeratin5; / DDD; / DDD1; / EBS2; / epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; / K2C5_HUMAN; / K5; / keratin 5; / epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types; / Keratin 5; / Keratin; / keratin complex 2, basic, gene 5; / keratin, type II cytoskeletal 5; / Keratin-5; / Keratin5; / KRT 5; / Krt5; / KRT5A; / type II cytoskeletal 5; / Type-II keratin Kb5; / CK 6A; / CK 6B; / CK 6C; / CK 6D; / CK 6E; / CK-6B; / CK-6C; / CK-6E; / Cytokeratin 6a; / Cytokeratin 6B; / Cytokeratin 6C; / Cytokeratin 6D; / Cytokeratin 6E; / Cytokeratin-6B; / Cytokeratin-6C; / Cytokeratin-6E; / K2C6C_HUMAN; / K6a keratin; / K6b keratin; / K6C; / K6c keratin; / K6d keratin; / K6e keratin; / Keratin; / Keratin K6h; / Keratin type II cytoskeletal 6A; / Keratin type II cytoskeletal 6B; / Keratin type II cytoskeletal 6C; / Keratin type II cytoskeletal 6D; / Keratin type II cytoskeletal 6E; / Keratin-6C; / KRT6A; / KRT6B; / KRT6C; / KRT6D; / KRT6E; / type II cytoskeletal 6C; / Type-II keratin Kb12; / 58 kDa Cytokeratin antibody; / CK-5 antibody; / CK5 antibody; / Cytokeratin-5 antibody; / Cytokeratin5 antibody; / DDD antibody; / DDD1 antibody; / EBS2 antibody; / epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody; / K2C5_HUMAN antibody; / K5 antibody; / keratin 5; / epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types; / antibody; / Keratin 5 antibody; / Keratin antibody; / keratin complex 2, basic, gene 5 antibody; / keratin, type II cytoskeletal 5 antibody; / Keratin-5 antibody; / Keratin5 antibody; / KRT 5 antibody; / Krt5 antibody; / KRT5A antibody; / type II cytoskeletal 5 antibody; / Type-II keratin Kb5 antibody
Species	Human
Gene ID	3852
Uniprot	P13647;P02538;P04259;P48668
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasmic, Membranous
Tissue Expression	Expressed in corneal epithelium (at protein level).
Signaling_pathway
Research Areas	>>Signal Transduction; / >>Cytoskeleton / ECM; / >>Cytoskeleton; / >>Intermediate Filaments; / >>Class I; / >>Cytokeratins
Function	Function: Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity). Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

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Cytokeratin 5/6 [ABT-CK5/052] mouse mAb

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Cytokeratin 5/6 [ABT-CK5/052] mouse mAb

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