Price range: $268.00 through $328.00
Background:
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],
| Size | 50μL/100μL |
| Target | PLK1 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB |
| MW(Calculated) | |
| MW(Observed) | 62kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC1:50-200; WB 1:1000-2000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Monoclonal |
| Clone Number | 6E4 |
| Immunogen | Synthesized peptide derived from human PLK1 |
| Specificity | This antibody detects endogenous levels of PLK1 at Human, Mouse,Rat |
| Gene Name | PLK1 PLK |
| Protein Name | PLK1 |
| Other Name | Serine/threonine-protein kinase PLK1; / Polo-like kinase 1; / PLK-1; / Serine/threonine-protein kinase 13; / STPK13 |
| Species | Human |
| Gene ID | 5347 |
| Uniprot | P53350, |
| Species.1 | Mouse |
| Gene ID.1 | 18817 |
| Uniprot.1 | Q07832, |
| Species.2 | Rat |
| Gene ID.2 | 25515 |
| Uniprot.2 | Q62673 |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Midbody . localization at the centrosome starts at the G1/S transition (PubMed:24018379). During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1. Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle. Localization to the centrosome is required for S phase progression (PubMed:24018379). Colocalizes with HSF1 at the spindle poles during prometaphase (PubMed:18794143). . |
| Tissue Expression | Placenta and colon. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>Cell cycle; / >>Oocyte meiosis; / >>Progesterone-mediated oocyte maturation |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,developmental stage:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.,enzyme regulation:Activated by serine and threonine phosphorylation.,Function:Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.,induction:By growth-stimulating agents.,PTM:Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.,PTM:Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 POLO box domains.,subunit:Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A.,tissue specificity:Placenta and colon., |
