Price range: $288.00 through $368.00
Background:The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Cyclin B1 Phospho Ser126 |
| Reactivity | Human |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 48kD |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | IHC 1:500-1:2000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0070R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | This antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Ser126.This antibody does not recognize phosphorylated at other sites. |
| Gene Name | CCNB1 CCNB |
| Protein Name | G2/mitotic-specific cyclin-B1 |
| Other Name | CCNB1; / CCNB; / G2/mitotic-specific cyclin-B1 |
| Species | Human |
| Gene ID | 891 |
| Uniprot | P14635, |
| Species.1 | Mouse |
| Gene ID.1 | 268697 |
| Uniprot.1 | P24860, |
| Species.2 | Rat |
| Gene ID.2 | 25203 |
| Uniprot.2 | P30277 |
| Cellular Localization | Cytoplasm, Nuclear |
| Tissue Expression | Breast adenocarcinoma,Lung,Placenta, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cell growth and death >> p53 signaling pathway;Cellular Processes >> Cell growth and death >> Cellular senescence;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway |
| Research Areas | |
| Function | developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,Function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase., |
