Product details

Background：
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor , which activates a signaling pathway for cell proliferation , differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers , and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Sep 2011] ,

Specifications

Size	50μL/100μL
Target	TGFα
Reactivity	Human, Mouse
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	17kD
MW(Observed)	19kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1724R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	TGFA
Protein Name	Protransforming growth factor alpha
Other Name	TGFA; / Protransforming growth factor alpha
Species	Human
Gene ID	7039
Uniprot	P01135,
Species.1	Mouse
Gene ID.1	21802
Uniprot.1	P48030,
Species.2	Rat
Gene ID.2	24827
Uniprot.2	P01134
Cellular Localization	[Transforming growth factor alpha]: Secreted, extracellular space.; [Protransforming growth factor alpha]: Cell membrane; Single-pass type I membrane protein.
Tissue Expression	Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
Signaling_pathway	Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>EGFR tyrosine kinase inhibitor resistance; / >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>PI3K-Akt signaling pathway; / >>Estrogen signaling pathway; / >>Pathways in cancer; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Pancreatic cancer; / >>Glioma; / >>Prostate cancer; / >>Non-small cell lung cancer; / >>Hepatocellular carcinoma
Function	Function:TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.,similarity:Contains 1 EGF-like domain.,subunit:Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2.,

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U-Blot® TGF-α Rabbit mAb

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U-Blot® TGF-α Rabbit mAb

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