SKU: WR5978 Categories: ,

U-Blot® Histone H4 (Acetyl Lys12) Rabbit mAb

Price range: $288.00 through $368.00

SKU: WR5978-50
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Product details

Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A , H2B , H3 , and H4) form an octamer , around which approximately 146 bp of DNA is wrapped in repeating units , called nucleosomes. The linker histone , H1 , interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq , Aug 2015] ,

Specifications

Size50μL/100μL
TargetHistone H4 L-Lactyl Lys12
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, ELISA
MW(Calculated)11kD
MW(Observed)11kD
Host SpeciesRabbit
IsotypeIgG, Kappa
Conjugate/Modificationlactylate
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year (Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM2041R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameHIST1H4A/HIST1H4B/HIST1H4C/HIST1H4D/HIST1H4E/HIST1H4F/HIST1H4H/HIST1H4I/HIST1H4J/HIST1H4K/HIST1H4L/HIST2H4A/HIST2H4B/HIST4H4
Protein NameHistone H4
Other NameHIST1H4A; / H4/A; / H4FA; / HIST1H4B; / H4/I; / H4FI; / HIST1H4C; / H4/G; / H4FG; / HIST1H4D; / H4/B; / H4FB; / HIST1H4E; / H4/J; / H4FJ; / HIST1H4F; / H4/C; / H4FC; / HIST1H4H; / H4/H; / H4FH; / HIST1H4I; / H4/M; / H4FM; / HIST1H4J; / H4/E; / H4FE; / HIST1H4K; / H4/D; / H4FD; / HIST1H4L; / H4/K; / H4FK
SpeciesHuman
Gene ID121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
UniprotP62805,
Species.1Mouse
Gene ID.1100041230
Uniprot.1P62806,
Species.2Rat
Gene ID.2100360950
Uniprot.2P62804
Cellular LocalizationNucleus. Chromosome.
Tissue ExpressionB-cell lymphoma ,Bone marrow ,Brain ,Clones donated by HIP ,Corpus call
Signaling_pathwayOrganismal Systems >> Immune system >> Neutrophil extracellular trap formation;Human Diseases >> Immune disease >> Systemic lupus erythematosus
Research Areas>>Neutrophil extracellular trap formation; / >>Alcoholism; / >>Viral carcinogenesis; / >>Systemic lupus erythematosus
FunctionFunction:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin , limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation , DNA repair , DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones , also called histone code , and nucleosome remodeling. ,PTM:L-Lactylation at Lys-6 , Lys-9 , Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin. ,PTM:Citrullination at Arg-4 by PADI4 impairs methylation. ,PTM:Monomethylated , dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. ,PTM:Monomethylation at Arg-4 by PRMT1 favors L-Lactylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6. ,PTM:Sumoylated , which is associated with transcriptional repression. ,PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. ,similarity:Belongs to the histone H4 family. ,subunit:The nucleosome is a histone octamer containing two molecules each of H2A , H2B , H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. ,
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