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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Mouse mAb/U-Blot® RNA pol II CTD Ser2ph / Ser5ph Monoclonal Antibody
SKU: WM2980 Categories: ,

U-Blot® RNA pol II CTD Ser2ph / Ser5ph Monoclonal Antibody

UcallM

$999.00

SKU: WM2980-10
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Product details

Background:SIRT1 ( Sirtuin 1 , SIR2L1 ) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates ( NBS1 , p53 , Ku70, etc. ) as well as from Histone H4 Lys16 . SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans .

Specifications

Target--
ReactivityHuman
ApplicationsChIP; ChIP-Seq; WB; IF; ICC
MW(Calculated)240 kDa
Host SpeciesMouse
IsotypeIgG1, k
Recommended Dilution RatioApplications Validated by Active Motif: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 - 10 µg each ICC/IF: 5 µg/ml dilution WB*: 0.5 - 2 µl/ml dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here . *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
FormulationPurified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
PurificationProtein A Chromatography
StorageSome products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Concentration--
ClonalityMonoclonal
Clone Number1A12G10
ImmunogenThis antibody was raised against a synthetic peptide containing phospho-serine 2 and phospho-serine 5 of the CTD of human RNA Polymerase II.
Specificity--
Gene Name--
Other NameRPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
Gene ID--
Cellular Localization--
Function--
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