Price range: $268.00 through $328.00
Background:Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | MOES |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 68kD |
| MW(Observed) | 78kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1220R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MSN |
| Protein Name | MOES |
| Other Name | |
| Species | Human |
| Gene ID | 4478 |
| Uniprot | P26038, |
| Species.1 | Mouse |
| Gene ID.1 | 17698 |
| Uniprot.1 | P26041, |
| Species.2 | Rat |
| Gene ID.2 | 81521 |
| Uniprot.2 | O35763 |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasm, cytoskeleton . Apical cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, microvillus . Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By similarity). Localizes at the uropods of T lymphoblasts. . |
| Tissue Expression | In all tissues and cultured cells studied. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Leukocyte transendothelial migration |
| Research Areas | >>Tight junction; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Measles; / >>Proteoglycans in cancer |
| Function | Function:Probably involved in connections of major cytoskeletal structures to the plasma membrane.,PTM:Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.,similarity:Contains 1 FERM domain.,subcellular location:Phosphorylated form is enriched in microvilli-like structures at apical membrane.,subunit:In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation (By similarity). Binds SLC9A3R1.,tissue specificity:In all tissues and cultured cells studied., |
