Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016],
| Size | 50μL/100μL |
| Target | RPA1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 189kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | RPA1 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | POLR1A |
| Protein Name | DNA-directed RNA polymerase I subunit RPA1 (RNA polymerase I subunit A1) (A190) (DNA-directed RNA polymerase I largest subunit) (DNA-directed RNA polymerase I subunit A) (RNA polymerase I 194 kDa subunit) (RPA194) |
| Other Name | |
| Species | Human |
| Gene ID | 25885 |
| Uniprot | O95602, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O35134, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | O54889 |
| Cellular Localization | Nucleus, nucleolus . Chromosome . |
| Tissue Expression | Colon,Skin,Uterus, |
| Signaling_pathway | |
| Research Areas | >>RNA polymerase |
| Function | Catalytic activity:Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).,Function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.,PTM:Phosphorylated.,similarity:Belongs to the RNA polymerase beta' chain family.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with MYO1C., |
