SKU: WP10975 Categories: ,

AGS3 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP10975-100
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Product details

Background:

G-protein signaling modulators (GPSMs) play diverse functional roles through their interaction with G-protein subunits. This gene encodes a receptor-independent activator of G protein signaling, which is one of several factors that influence the basal activity of G-protein signaling systems. The protein contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],

Specifications

Size50μL/100μL
TargetAGS3
ReactivityHuman, Mouse, Rat
ApplicationsIHC, IF, ELISA
MW(Calculated)72kD
MW(Observed)
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from the Internal region of human AGS3.
SpecificityAGS3 Polyclonal Antibody detects endogenous levels of AGS3 protein.
Gene NameGPSM1
Protein NameG-protein-signaling modulator 1
Other NameGPSM1; / AGS3; / G-protein-signaling modulator 1; / Activator of G-protein signaling 3
SpeciesHuman
Gene ID26086
UniprotQ86YR5,
Species.1Mouse
Gene ID.167839
Uniprot.1Q6IR34,
Species.2Rat
Gene ID.2246254
Uniprot.2Q9R080
Cellular LocalizationCytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue ExpressionExpressed in intestinal cells.
Signaling_pathway
Research Areas>>Cocaine addiction
FunctionDomain:The GoLoco domains mediate interaction with G(i/o) alpha (By similarity). The GoLoco domains are essential for the GDI activity toward G(i/o) alpha.,Function:Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.,PTM:Phosphorylation regulates interaction with G(i/o) alpha.,similarity:Belongs to the GPSM family.,similarity:Contains 4 GoLoco domains.,similarity:Contains 9 TPR repeats.,subunit:Interacts with GNAI1, GNAI2 and GNAI3 preferentially in their GDP-bound state. May also interact with GNAO1. Interacts with STK11/LKB1 and MACF1 (By similarity). Interacts with INSC/inscuteable and FRMPD1.,tissue specificity:Expressed in intestinal cells.,
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