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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/BAF250b Polyclonal Antibody
SKU: WP11235 Categories: ,

BAF250b Polyclonal Antibody

Ucallm_WP11235-50

Price range: $268.00 through $328.00

SKU: WP11235-100
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Product details

Background:

This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2012],

Specifications

Size50μL/100μL
TargetBAF250b
ReactivityHuman, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)170kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human BAF250B. AA range:1371-1420
SpecificityBAF250b Polyclonal Antibody detects endogenous levels of BAF250b protein.
Gene NameARID1B
Protein NameAT-rich interactive domain-containing protein 1B
Other NameARID1B; / BAF250B; / DAN15; / KIAA1235; / OSA2; / AT-rich interactive domain-containing protein 1B; / ARID domain-containing protein 1B; / BRG1-associated factor 250b; / BAF250B; / BRG1-binding protein hELD/OSA1; / Osa homolog 2; / hOsa2; / p250R
SpeciesHuman
Gene ID57492
UniprotQ8NFD5
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus .
Tissue ExpressionWidely expressed with high levels in heart, skeletal muscle and kidney.
Signaling_pathwayHuman Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Genetic Information Processing >> Chromosome >> ATP-dependent chromatin remodeling
Research Areas>>Thermogenesis; / >>Hepatocellular carcinoma
FunctionCaution:It is uncertain whether Met-1 or Met-59 is the initiator.,Function:Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically.,similarity:Contains 1 ARID domain.,subunit:Component of SWI/SNF chromatin remodeling complexes, in some of which it can be mutually exclusive with ARID1A/BAF250A. Component of the BAF (SWI/SNF-A) complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of the SWI/SNF-B (PBAF) complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PB1/BAF180, ARID2/BAF200, ARID1A/BAF250A or ARID1B/BAF250B and actin. Component of a SWI/SNF-like EPAFb complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47, ACTL6A/BAF53A, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, SMARCC1/BAF155, SMARCC2/BAF170, ARID1B/BAF250B, MLLT1/ENL and actin. Component of a SWI/SNF-like complex containing ARID1A/BAF250A and ARID1B/BAF250B. Interacts through its C-terminus with SMARCA2/BRM and SMARCA4/BRG1. Interacts with SMARCC1/BAF155.,tissue specificity:Widely expressed with high levels in heart, skeletal muscle and kidney.,
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