Price range: $268.00 through $328.00
Background:
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008],
| Target | BLM |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 159kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | |
| Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications. |
| Composition | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification Process | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage Condition | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Bloom Syndrome. AA range:65-114 |
| Sequence | |
| Specificity | BLM Polyclonal Antibody detects endogenous levels of BLM protein. |
| Gene Name | BLM |
| Protein Name | Bloom syndrome protein |
| Alias | BLM;RECQ2;RECQL3;Bloom syndrome protein;DNA helicase;RecQ-like type 2;RecQ2;RecQ protein-like 3 |
| Organism-1 | Human |
| Gene ID-1 | 641 |
| SwissProt-1 | P54132 |
| Organism-2 | Mouse |
| Gene ID-2 | |
| SwissProt-2 | O88700 |
| Organism-3 | |
| Gene ID-3 | |
| SwissProt-3 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Cellular Localization | Nucleus . Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner. |
