Price range: $268.00 through $328.00
Background:
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | CARD 10 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 120kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CARD10. AA range:481-530 |
| Specificity | CARD 10 Polyclonal Antibody detects endogenous levels of CARD 10 protein. |
| Gene Name | CARD10 |
| Protein Name | Caspase recruitment domain-containing protein 10 |
| Other Name | CARD10; / CARMA3; / Caspase recruitment domain-containing protein 10; / CARD-containing MAGUK protein 3; / Carma 3 |
| Species | Human |
| Gene ID | 29775 |
| Uniprot | Q9BWT7, |
| Species.1 | Mouse |
| Gene ID.1 | 105844 |
| Uniprot.1 | P58660 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway |
| Research Areas | >>NF-kappa B signaling pathway |
| Function | Caution:Supposed to contain a SH3, a PDZ and a guanylate kinase-like domain. But none of these 3 domains are detected by PROSITE, Pfam or SMART.,Function:Activates NF-kappa-B via BCL10 and IKK.,similarity:Contains 1 CARD domain.,subunit:CARD10 and BCL10 bind to each other by CARD-CARD interaction. They both participate in a complex with MALT1, where MALT1 binds to BCL10.,tissue specificity:Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney., |
