Price range: $268.00 through $328.00
Background:
The phospholipase A2 enzyme family, including PLA2G4D, catalyze the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids (Chiba et al., 2004 [PubMed 14709560]).[supplied by OMIM, Jun 2009],
| Size | 50μL/100μL |
| Target | cPLA2-δ |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 92kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLA2G4D. AA range:531-580 |
| Specificity | cPLA2-δ Polyclonal Antibody detects endogenous levels of cPLA2-δ protein. |
| Gene Name | PLA2G4D |
| Protein Name | Cytosolic phospholipase A2 delta |
| Other Name | PLA2G4D; / Cytosolic phospholipase A2 delta; / cPLA2-delta; / Phospholipase A2 group IVD |
| Species | Human |
| Gene ID | 283748 |
| Uniprot | Q86XP0, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q50L43 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol . Membrane; Peripheral membrane protein; Cytoplasmic side . Translocates to perinuclear membranes that may correspond to endoplasmic reticulum or Golgi in a calcium-dependent fashion. . |
| Tissue Expression | Expressed in stratified squamous epithelia, such as those in skin and cervix, but not in other tissues (PubMed:14709560). Strongly expressed in the upper spinous layer of the psoriatic epidermis, expressed weakly and discontinuously in atopic dermatitis and mycosis fungoides, and not detected in the epidermis of normal skin (PubMed:14709560). |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway |
| Research Areas | >>Glycerophospholipid metabolism; / >>Ether lipid metabolism; / >>Arachidonic acid metabolism; / >>Linoleic acid metabolism; / >>alpha-Linolenic acid metabolism; / >>Metabolic pathways; / >>MAPK signaling pathway; / >>Ras signaling pathway; / >>Phospholipase D signaling pathway; / >>Necroptosis; / >>Vascular smooth muscle contraction; / >>VEGF signaling pathway; / >>Platelet activation; / >>Fc epsilon RI signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Glutamatergic synapse; / >>Serotonergic synapse; / >>Long-term depression; / >>Inflammatory mediator regulation of TRP channels; / >>GnRH signaling pathway; / >>Ovarian steroidogenesis; / >>Oxytocin signaling pathway; / >>Choline metabolism in cancer |
| Function | Catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.,Domain:The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).,enzyme regulation:Stimulated by cytosolic Ca(2+).,Function:Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Not arachidonic acid-specific but has linoleic acid-specific activity. May play a role in inflammation in psoriatic lesions.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PLA2c domain.,subcellular location:Translocates to membrane vesicles in a calcium-dependent fashion. Translocates to perinuclear regions upon ionomycin stimulation.,tissue specificity:Expressed in stratified squamous epithelia, such as those in skin and cervix, but not in other tissues. Strongly expressed in the upper spinous layer of the psoriatic epidermis, expressed weakly and discontinuously in atopic dermatitis and mycosis fungoides, and not detected in the epidermis of normal skin., |
