Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],
| Size | 50μL/100μL |
| Target | Cytochrome b5 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 15kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110 |
| Specificity | Cytochrome b5 Polyclonal Antibody detects endogenous levels of Cytochrome b5 protein. |
| Gene Name | CYB5A |
| Protein Name | Cytochrome b5 |
| Other Name | CYB5A; / CYB5; / Cytochrome b5; / Microsomal cytochrome b5 type A; / MCB5 |
| Species | Human |
| Gene ID | 1528 |
| Uniprot | P00167, |
| Species.1 | Mouse |
| Gene ID.1 | 109672 |
| Uniprot.1 | P56395, |
| Species.2 | Rat |
| Gene ID.2 | 64001 |
| Uniprot.2 | P00173 |
| Cellular Localization | [Isoform 1]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm. |
| Tissue Expression | Erythrocyte,Liver,Spleen,Uterus, |
| Signaling_pathway | |
| Research Areas | |
| Function | Disease:Defects in CYB5A are the cause of type IV hereditary methemoglobinemia [MIM:250790].,Function:Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.,similarity:Belongs to the cytochrome b5 family.,similarity:Contains 1 cytochrome b5 heme-binding domain., |
