Price range: $268.00 through $328.00
Background:
kinesin family member 11(KIF11) Homo sapiens This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Eg5 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 119kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human KIF11/Eg5. AA range:892-941 |
| Specificity | Eg5 Polyclonal Antibody detects endogenous levels of Eg5 protein. |
| Gene Name | KIF11 |
| Protein Name | Kinesin-like protein KIF11 |
| Other Name | KIF11; / EG5; / KNSL1; / TRIP5; / Kinesin-like protein KIF11; / Kinesin-like protein 1; / Kinesin-like spindle protein HKSP; / Kinesin-related motor protein Eg5; / Thyroid receptor-interacting protein 5; / TR-interacting protein 5; / TRIP-5 |
| Species | Human |
| Gene ID | 3832 |
| Uniprot | P52732, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q6P9P6 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Cytoplasm, cytoskeleton, spindle pole . |
| Tissue Expression | Brain,Epithelium,Lung, |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.,PTM:Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the kinesin-like protein family. BimC subfamily.,similarity:Contains 1 kinesin-motor domain.,subunit:Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5., |
