SKU: WP12169 Categories: ,

Eme1 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP12169-100
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Product details

Background:

This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009],

Specifications

Size50μL/100μL
TargetEme1
ReactivityHuman, Mouse
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)65kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; ELISA 1:5000; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from Eme1 . at AA range: 250-330
SpecificityEme1 Polyclonal Antibody detects endogenous levels of Eme1 protein.
Gene NameEME1
Protein NameCrossover junction endonuclease EME1
Other NameEME1; / MMS4; / Crossover junction endonuclease EME1; / MMS4 homolog; / hMMS4
SpeciesHuman
Gene ID146956
UniprotQ96AY2,
Species.1Mouse
Gene ID.1
Uniprot.1Q8BJW7
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus, nucleolus . Recruited to regions of DNA damage in S-phase cells.
Tissue ExpressionEpithelium,Placenta,
Signaling_pathway
Research Areas>>Homologous recombination; / >>Fanconi anemia pathway
Functioncofactor:Magnesium.,Function:Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.,similarity:Belongs to the EME1/MMS4 family.,subcellular location:Recruited to regions of DNA damage in S-phase cells.,subunit:May self-associate. Interacts with MUS81. Interacts with ERCC4 and FANCM.,
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