Product details

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],

Specifications

Target	Histone H4
Reactivity	Human, Mouse, Rat, Monkey
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	11kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Histone H4. AA range:6-55
Sequence
Specificity	Histone H4 Polyclonal Antibody detects endogenous levels of Histone H4 protein.
Gene Name	HIST1H4A/HIST1H4B/HIST1H4C/HIST1H4D/HIST1H4E/HIST1H4F/HIST1H4H/HIST1H4I/HIST1H4J/HIST1H4K/HIST1H4L/HIST2H4A/HIST2H4B/HIST4H4
Protein Name	Histone H4
Other Name	HIST1H4A; / H4/A; / H4FA; / HIST1H4B; / H4/I; / H4FI; / HIST1H4C; / H4/G; / H4FG; / HIST1H4D; / H4/B; / H4FB; / HIST1H4E; / H4/J; / H4FJ; / HIST1H4F; / H4/C; / H4FC; / HIST1H4H; / H4/H; / H4FH; / HIST1H4I; / H4/M; / H4FM; / HIST1H4J; / H4/E; / H4FE; / HIST1H4K; / H4/D; / H4FD; / HIST1H4L; / H4/K; / H4FK
Species	Human
Gene ID-1	121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Uniprot	P62805,
Species.1	Mouse
Gene ID-2	100041230
Uniprot.1	P62806,
Species.2	Rat
Gene ID-3	100360950
Uniprot.2	P62804
Organism-4
Gene ID-4
SwissProt-4
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],
Cellular Localization	Nucleus. Chromosome.
Tissue Expression	B-cell lymphoma,Bone marrow,Brain,Clones donated by HIP,Corpus call
Signaling_pathway	Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Human Diseases >> Immune disease >> Systemic lupus erythematosus
Research Areas	>>Neutrophil extracellular trap formation; / >>Alcoholism; / >>Viral carcinogenesis; / >>Systemic lupus erythematosus
Function	Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.,PTM:Citrullination at Arg-4 by PADI4 impairs methylation.,PTM:Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.,PTM:Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.,PTM:Sumoylated, which is associated with transcriptional repression.,PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.,similarity:Belongs to the histone H4 family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.,
RRID

Please login to post questions

U-Blot® Histone H4 Rabbit pAb

Product details

Specifications

About Us

Support

Order

Menu

U-Blot® Histone H4 Rabbit pAb

Product details

Specifications

Related products

U-Blot® HDAC2 Rabbit mAb

U-Blot® MMP7 Rabbit mAb

U-Blot® GSK3 β (Phospho Ser9) Rabbit mAb

U-Blot® CDK6 Rabbit mAb

U-Blot® PMS2 Rabbit mAb

U-Blot® Tuberin Rabbit mAb

Customer Reviews

Menu