Product details

Background:

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013],

Specifications

Target	ILK
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	42kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human ILK. AA range:212-261
Sequence
Specificity	ILK Polyclonal Antibody detects endogenous levels of ILK protein.
Gene Name	ILK ILK1 ILK2
Protein Name	Integrin-linked protein kinase
Other Name	ILK; / ILK1; / ILK2; / Integrin-linked protein kinase; / 59 kDa serine/threonine-protein kinase; / ILK-1; / ILK-2; / p59ILK
Species	Human
Gene ID-1	3611
Uniprot	Q13418,
Species.1	Mouse
Gene ID-2	16202
Uniprot.1	O55222,
Species.2	Rat
Gene ID-3	170922
Uniprot.2	Q99J82
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013],
Cellular Localization	Cell junction, focal adhesion . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere .
Tissue Expression	Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Organismal Systems >> Endocrine system >> PPAR signaling pathway;Human Diseases >> Cancer: specific types >> Endometrial cancer
Research Areas	>>PPAR signaling pathway; / >>Axon guidance; / >>Focal adhesion; / >>Bacterial invasion of epithelial cells; / >>Shigellosis; / >>Endometrial cancer
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:A PH-like domain is involved in phosphatidylinositol phosphate binding.,enzyme regulation:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.,Function:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.,PTM:Autophosphorylated on serine residues.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,similarity:Contains 5 ANK repeats.,subunit:Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1.,tissue specificity:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.,
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ILK Polyclonal Antibody

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ILK Polyclonal Antibody

Product details

Specifications

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