Price range: $268.00 through $328.00
Background:
SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | SPRE2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | 46kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human SPRE2 AA range: 68-118 |
| Specificity | This antibody detects endogenous levels of SPRE2 at Human/Mouse/Rat |
| Gene Name | SPRED2 |
| Protein Name | SPRE2 |
| Other Name | |
| Species | Human |
| Gene ID | 200734 |
| Uniprot | Q7Z698, |
| Species.1 | Mouse |
| Gene ID.1 | 114716 |
| Uniprot.1 | Q924S7, |
| Species.2 | Rat |
| Gene ID.2 | 305539 |
| Uniprot.2 | Q3C2P8 |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasm . Detected in the cytoplasm of the stratum spinosum cells, where it is associated with cytoplasmic vesicles that are supposed to be secretory granules. . |
| Tissue Expression | Expressed in liver, skin, small intestine, salivary gland and prostate. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.,PTM:Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination.,PTM:Ubiquitinated; leading to degradation by the proteasome.,similarity:Contains 1 KBD domain.,similarity:Contains 1 SPR (sprouty) domain.,similarity:Contains 1 WH1 domain.,subcellular location:Especially found in the cytoplasm of the stratum spinosum cells called membrane-coated granules.,subunit:Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers.,tissue specificity:Expressed in liver, skin, small intestine, salivary gland and prostate., |
