Price range: $268.00 through $328.00
Background:
The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | LCMT2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | 75kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human LCMT2 AA range: 298-348 |
| Specificity | This antibody detects endogenous levels of LCMT2 at Human/Mouse/Rat |
| Gene Name | LCMT2 KIAA0547 TYW4 |
| Protein Name | LCMT2 |
| Other Name | |
| Species | Human |
| Gene ID | 9836 |
| Uniprot | O60294, |
| Species.1 | Mouse |
| Gene ID.1 | 329504 |
| Uniprot.1 | Q8BYR1, |
| Species.2 | Rat |
| Gene ID.2 | 296098 |
| Uniprot.2 | Q5XIA3 |
| Cellular Localization | cytoplasm,cytosol, |
| Tissue Expression | |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.,pathway:tRNA modification; wybutosine-tRNA(Phe) biosynthesis.,similarity:Belongs to the methyltransferase superfamily. LCMT family.,subunit:Interacts with RNF144B/IBRDC2., |
