Fibrillarin Rabbit Monoclonal Antibody

Fibrillarin Rabbit Monoclonal Antibody

Background:This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence.

Category Content
Primary Antibody Fibrillarin Rabbit mAb:WR4102
Membrane 0.22 Nitrocellulose Membrane:W3N21
Protein Marker pre-staining standard protein Marker(10-180kDa):W2100
Lane Content
1 HL60 – Human promyelocytic leukemia cell
2 HUVEC – Human umbilical vein endothelial cell
3 Human CAF – Human cancer-associated fibroblast
4 HCT116 – Human colorectal carcinoma
5 A549 – Human lung carcinoma
6 Jurkat – Human T lymphocyte leukemia
7 HT22 – Mouse hippocampal neuronal
8 Thp-1 – Human monocytic leukemia
9 HK-2 – Human proximal tubular epithelial
10 PC-12 – Rat adrenal pheochromocytoma
11 3T3-L1 – Mouse embryonic fibroblast cells
12 PC-3 – Human prostate adenocarcinoma
13 U-14 – Mouse cervical carcinoma
14 293F – HEK293 derivative, adapted for suspension culture
Step Content
Experimental Operations Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
Predicted band size Observed band size
34 kDa 34 kDa

Elevate your nucleolar biology and RNA processing research with the Fibrillarin Rabbit Monoclonal Antibody (WR4102)—a Google-optimized, rigorously validated tool for specific Fibrillarin detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, A549, etc.), this antibody delivers perfect band alignment (34 kDa predicted/observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated Goat anti-Rabbit detection system.

Ideal for studying nucleolar small nuclear ribonucleoprotein (snRNP) complexes, preribosomal RNA processing, dense fibrillar component (DFC) localization, or Fibrillarin’s RNA-binding domain function, this trusted Fibrillarin Rabbit Monoclonal Antibody boosts assay reproducibility. Order WR4102 today to advance your Fibrillarin-focused experiments with a SEO-verified, high-performance research tool tailored for ribosome biology and nucleolar function studies.

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