Name: U-Blot® Fibrillarin Rabbit mAb
SKU: WR4102
Price: 268 USD
Availability: InStock

U-Blot® Fibrillarin Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4102 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

Western blot analysis of lysates from 3T3-L1 cells, using Fibrillarin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4102 Western blot analysis of lysates from 3T3-L1 cells, using Fibrillarin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4102 Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded Rat liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4102 Immunohistochemical analysis of paraffin-embedded Rat liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Price range: $268.00 through $328.00

Size

100μl

50μl

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SKU: WR4102-50

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Product details

Background:This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Specifications

Size	50μL/100μL
Target	FBL
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	34kD
MW(Observed)	34kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0195R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	FBL FIB1 FLRN
Protein Name	rRNA 2'-O-methyltransferase fibrillarin (34 kDa nucleolar scleroderma antigen)
Other Name
Species	Human
Gene ID	2091
Uniprot	P22087,
Species.1	Mouse
Gene ID.1	100044829
Uniprot.1	P35550,
Species.2	Rat
Gene ID.2	292747
Uniprot.2	P22509
Cellular Localization	Nucleus
Tissue Expression
Signaling_pathway
Research Areas
Function	S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins . Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA . Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes . U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity . Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus .