β-Tubulin Monoclonal Antibody [5G3], AbFluor 555 Conjugated
Ucallm_WM0954-50
Price range: $268.00 through $328.00
Product details
Background:
tubulin beta 3 class III(TUBB3) Homo sapiens This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010],
Specifications
| Size | 50μL/100μL |
| Target | Tubulin β |
| Reactivity | Human, Mouse, Rat, Mk, Dg, Ch, Hamster, sheep, Insect, Yeast |
| Applications | WB, IF, IHC |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugate/Modification | AbFluor 555 |
| Recommended Dilution Ratio | Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:IHC 1:200; IF 1:200. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage | Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark. |
| Concentration | 1mg/ml |
| Clonality | Monoclonal |
| Clone Number | 5G3 |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | β-Tubulin Monoclonal Antibody(5G3) AbFluor™ 555 Conjugated specially designed for your Immunofluorescence analysis. |
| Gene Name | TUBB3 |
| Protein Name | Tubulin beta-3 chain |
| Other Name | TUBB3 |
| Species | Human |
| Gene ID | 10381 |
| Uniprot | Q13509 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytoskeleton . Cell projection, growth cone . Cell projection, lamellipodium . Cell projection, filopodium . |
| Tissue Expression | Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases |
| Research Areas | >>Phagosome; / >>Gap junction; / >>Alzheimer disease; / >>Parkinson disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Pathogenic Escherichia coli infection; / >>Salmonella infection |
| Function | Domain:The highly acidic C-terminal region may bind cations such as calcium.,Function:Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.,Function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,polymorphism:Genetic variations in MC1R are associated with variation in skin/hair/eye pigmentation type 2 (SHEP2) [MIM:266300]. Hair, eye and skin pigmentation are among the most visible examples of human phenotypic variation, with a broad normal range that is subject to substantial geographic stratification. In the case of skin, individuals tend to have lighter pigmentation with increasing distance from the equator. By contrast, the majority of variation in human eye and hair color is found among individuals of European ancestry, with most other human populations fixed for brown eyes and black hair.,polymorphism:Variations in MC1R are linked to the degree of skin pigmentation (Types I-IV). Type I skin the most lightly pigmented and type IV the most dark pigmented. Partial loss-of-function mutations are associated with fair skin, poor tanning and increased skin cancer risk.,similarity:Belongs to the G-protein coupled receptor 1 family.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.,tissue specificity:Melanocytes and corticoadrenal tissue., |
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