Name: 14-3-3 β/ζ Polyclonal Antibody
SKU: WP10843
Price: 268 USD
Availability: InStock

Product details

Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],

Specifications

Size	50μL/100μL
Target	14-3-3 β/ζ
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	24kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; ELISA 1:20000; IF 1:50-200
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human YWHAZ. AA range:151-200
Specificity	14-3-3 β/ζ Polyclonal Antibody detects endogenous levels of 14-3-3 β/ζ protein.
Gene Name	YWHAB/YWHAZ
Protein Name	14-3-3 protein beta/alpha/14-3-3 protein zeta/delta
Other Name	YWHAZ; / 14-3-3 protein zeta/delta; / Protein kinase C inhibitor protein 1; / KCIP-1; / YWHAB; / 14-3-3 protein beta/alpha; / Protein 1054; / Protein kinase C inhibitor protein 1; / KCIP-1
Species	Human
Gene ID	7534;7529
Uniprot	P63104;P31946,
Species.1	Mouse
Gene ID.1	22631;54401,
Uniprot.1
Species.2	Rat
Gene ID.2	25578;56011
Uniprot.2	P63102;P35213
Cellular Localization	Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.
Tissue Expression	B-cell lymphoma,Bone marrow
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cell cycle;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>Cell cycle; / >>Oocyte meiosis; / >>PI3K-Akt signaling pathway; / >>Hippo signaling pathway; / >>Hepatitis C; / >>Hepatitis B; / >>Viral carcinogenesis
Function	Caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,Function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to stage I to stage IV melanosomes.,subunit:Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with PCTK1 and BSPRY (By similarity). Interacts with WEE1 (C-terminal) (By similarity). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm (By similarity). Interacts with Thr-phosphorylated ITGB2 (By similarity). Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional acivity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with AANAT ('Thr-31' phosphorylated form); the interaction modulates AANAT enzymatic activity through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT ('Ser-205' phosphorylated form), can bind the other YWHAZ monomer with similar effect. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization.,