SKU: WP10842 Categories: ,

14-3-3 β Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP10842-100
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Product details

Background:

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008],

Specifications

Target14-3-3 β
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)28kD
Host SpeciesRabbit
IsotypeIgG
Conjugate
ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
CompositionLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification ProcessThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human 14-3-3 beta. AA range:41-90
Sequence
Specificity14-3-3 β Polyclonal Antibody detects endogenous levels of 14-3-3 β protein.
Gene NameYWHAB
Protein Name14-3-3 protein beta/alpha
AliasYWHAB;14-3-3 protein beta/alpha;Protein 1054;Protein kinase C inhibitor protein 1;KCIP-1
Organism-1Human
Gene ID-17529
SwissProt-1P31946
Organism-2Mouse
Gene ID-254401
SwissProt-2Q9CQV8
Organism-3Rat
Gene ID-356011
SwissProt-3P35213
Organism-4
Gene ID-4
SwissProt-4
Cellular LocalizationCytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.; Vacuole membrane . (Microbial infection) Upon infection with Chlamydia trachomatis, this protein is associated with the pathogen-containing vacuole membrane where it colocalizes with IncG. .
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