SKU: WP0313 Categories: ,

14-3-3-pan (Acetyl Lys51/49) Polyclonal Antibody

Price range: $288.00 through $368.00

SKU: WP0313-100
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Product details

Background:

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008],

Specifications

Target14-3-3-pan
ReactivityHuman, Mouse, Rat
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)30kD
Host SpeciesRabbit
IsotypeIgG
Conjugate
ModificationAcetyl
Recommended Dilution RatioWB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
CompositionLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification ProcessThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized acetyl-peptide derived from human 14-3-3-pan around the acetylation site of K51.
Sequence
SpecificityAcetyl-14-3-3-pan (K51/49) Polyclonal Antibody detects endogenous levels of 14-3-3-pan around the acetylation site of K51 protein.
Gene NameYWHAB/YWHAG/YWHAQ/YWHAZ/SFN
Protein Name14-3-3 protein beta/alpha/14-3-3 protein gamma/14-3-3 protein theta/14-3-3 protein zeta/delta/14-3-3 protein sigma
AliasYWHAB;14-3-3 protein beta/alpha;Protein 1054;Protein kinase C inhibitor protein 1;KCIP-1;YWHAG;14-3-3 protein gamma;Protein kinase C inhibitor protein 1;KCIP-1;YWHAQ;14-3-3 protein theta;14-3-3 protein T-cell;14-3-3 protein tau;Protein HS1;YWHAZ;14-3-3 protein zeta/delta;Protein kinase C inhibitor protein 1;KCIP-1;SFN;HME1;14-3-3 protein sigma;Epithelial cell marker protein 1;Stratifin
Organism-1Human
Gene ID-17529
SwissProt-1P31946
Organism-2Mouse
Gene ID-254401
SwissProt-2Q9CQV8
Organism-3Rat
Gene ID-356011
SwissProt-3P35213
Organism-4
Gene ID-4
SwissProt-4
Cellular LocalizationCytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.; Vacuole membrane . (Microbial infection) Upon infection with Chlamydia trachomatis, this protein is associated with the pathogen-containing vacuole membrane where it colocalizes with IncG. .
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