Name: 4E-BP1 Monoclonal Antibody
SKU: WM0007
Price: 268 USD
Availability: InStock

Product details

Background:
eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	4E-BP1
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)	13kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	12D9
Immunogen	Purified recombinant fragment of 4E-BP1 expressed in E. Coli.
Specificity	4E-BP1 Monoclonal Antibody detects endogenous levels of 4E-BP1 protein.
Gene Name	EIF4EBP1
Protein Name	Eukaryotic translation initiation factor 4E-binding protein 1
Other Name	EIF4EBP1; / Eukaryotic translation initiation factor 4E-binding protein 1; / 4E-BP1; / eIF4E-binding protein 1; / Phosphorylated heat- and acid-stable protein regulated by insulin 1; / PHAS-I
Species	Human
Gene ID	1978
Uniprot	Q13541
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus
Tissue Expression	Colon,Epithelium,Lung,Placenta,Platelet,
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>EGFR tyrosine kinase inhibitor resistance; / >>ErbB signaling pathway; / >>HIF-1 signaling pathway; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Cellular senescence; / >>Insulin signaling pathway; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Herpes simplex virus 1 infection; / >>Chemical carcinogenesis - receptor activation; / >>Acute myeloid leukemia; / >>Choline metabolism in cancer
Function	Function:Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.,PTM:Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eIF4E-binding protein family.,subunit:Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.,

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4E-BP1 Monoclonal Antibody

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4E-BP1 Monoclonal Antibody

Product details

Specifications

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