53BP1 Polyclonal Antibody
Ucallm_WP10862-50
Price range: $268.00 through $328.00
Product details
Background:
function:May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage., PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding., PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation., similarity:Contains 2 BRCT domains., subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at ‘Lys-20’ is required for efficient localization to double strand breaks., subunit:Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on ‘Ser-139’. Interacts with histone H4 that has been dimethylated at ‘Lys-20’. Has low affinity for histone H4 containing monomethylated ‘Lys-20’. Does not bind histone H4 containing unmethylated or trimethylated ‘Lys-20’. Has low affinity for histone H3 that has been dimethylated on ‘Lys-79’. Has very low affinity for histone H3 that has been monomethylated on ‘Lys-79’ (in vitro). Does not bind unmethylated histone H3.,
Specifications
| Size | 50μL/100μL |
| Target | 53BP1 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 214kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TP53BP1. AA range:1744-1793 |
| Specificity | 53BP1 Polyclonal Antibody detects endogenous levels of 53BP1 protein. |
| Gene Name | TP53BP1 |
| Protein Name | Tumor suppressor p53-binding protein 1 |
| Other Name | TP53BP1; / Tumor suppressor p53-binding protein 1; / 53BP1; / p53-binding protein 1; / p53BP1 |
| Species | Human |
| Gene ID | 7158 |
| Uniprot | Q12888, |
| Species.1 | Mouse |
| Gene ID.1 | 27223 |
| Uniprot.1 | P70399 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus |
| Tissue Expression | Cerebellum,Cervix,Epithelium,Myeloid leukemia cell,Skeletal muscle, |
| Signaling_pathway | Organismal Systems >> Immune system >> NOD-like receptor signaling pathway |
| Research Areas | >>NOD-like receptor signaling pathway |
| Function | Function:May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.,PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.,PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.,similarity:Contains 2 BRCT domains.,subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.,subunit:Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Has low affinity for histone H4 containing monomethylated 'Lys-20'. Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20'. Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3., |
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