SKU: WP9935 Categories: ,

A-Raf (phospho Tyr302) Polyclonal Antibody

Price range: $288.00 through $368.00

SKU: WP9935-100
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Product details

Background:

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012],

Specifications

TargetA-Raf
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)68kD
Host SpeciesRabbit
IsotypeIgG
Conjugate
ModificationPhospho
Recommended Dilution RatioWB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
CompositionLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification ProcessThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302. AA range:276-325
Sequence
SpecificityPhospho-A-Raf (Y302) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at Y302.
Gene NameARAF
Protein NameSerine/threonine-protein kinase A-Raf
AliasARAF;ARAF1;PKS;PKS2;Serine/threonine-protein kinase A-Raf;Proto-oncogene A-Raf;Proto-oncogene A-Raf-1;Proto-oncogene Pks
Organism-1Human
Gene ID-1369
SwissProt-1P10398
Organism-2Mouse
Gene ID-211836
SwissProt-2P04627
Organism-3Rat
Gene ID-364363
SwissProt-3P14056
Organism-4
Gene ID-4
SwissProt-4
Cellular Localizationintracellular, mitochondrion, cytosol,
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