AAK1 Polyclonal Antibody
Ucallm_WP10874-50
Price range: $268.00 through $328.00
Product details
Background:
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | AAK1 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 94kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AAK1. AA range:271-320 |
| Specificity | AAK1 Polyclonal Antibody detects endogenous levels of AAK1 protein. |
| Gene Name | AAK1 |
| Protein Name | AP2-associated protein kinase 1 |
| Other Name | AAK1; / KIAA1048; / AP2-associated protein kinase 1; / Adaptor-associated kinase 1 |
| Species | Human |
| Gene ID | 22848 |
| Uniprot | Q2M2I8, |
| Species.1 | Mouse |
| Gene ID.1 | 269774 |
| Uniprot.1 | Q3UHJ0, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P0C1X8 |
| Cellular Localization | Cell membrane; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse, presynapse . Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells. . |
| Tissue Expression | Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain. |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Stimulated by clathrin.,Function:Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Active when found in clathrin-coated pits at the plasma membrane.,subunit:Interacts with alpha-adaptin and AP-2., |
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