Specifications
| Size | 50μL/100μL |
| Target | ACE2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 120-135kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-200; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Monoclonal |
| Clone Number | 2C1 |
| Immunogen | Synthesized peptide derived from human ACE2 |
| Specificity | This antibody detects endogenous levels of ACE2 at Human, Mouse,Rat |
| Gene Name | ACE2 UNQ868/PRO1885 |
| Protein Name | ACE2 |
| Other Name | Angiotensin-converting enzyme 2; / ACE-related carboxypeptidase; / Angiotensin-converting enzyme homolog; / ACEH; / Metalloprotease MPROT15; / [Cleaved into: Processed angiotensin-converting enzyme 2] |
| Species | Human |
| Gene ID | 59272 |
| Uniprot | Q9BYF1, |
| Species.1 | Mouse |
| Gene ID.1 | 70008 |
| Uniprot.1 | Q8R0I0, |
| Species.2 | Rat |
| Gene ID.2 | 302668 |
| Uniprot.2 | Q5EGZ1 |
| Cellular Localization | [Processed angiotensin-converting enzyme 2]: Secreted .; Cell membrane; Single-pass type I membrane protein . Cytoplasm . Cell projection, cilium . Apical cell membrane . Detected in both cell membrane and cytoplasm in neurons. .; [Isoform 2]: Apical cell membrane . |
| Tissue Expression | Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level) (PubMed:15141377). Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level) (PubMed:15141377). Expressed in the renal proximal tubule and the small intestine (at protein level) (PubMed:18424768). Expressed in heart, kidney, testis, and gastrointestinal system (at protein level) (PubMed:10969042, PubMed:10924499, PubMed:15231706, PubMed:12459472, PubMed:15671045, PubMed:32715618, PubMed:32170560). In lung, expressed at low levels in some alveolar type 2 cells, the expression seems to be individual-specific (at protein level) (PubMed:32425701, PubMed:15141377, PubMed:32715618, PubMed:32170560, PubMed:33432184). Expressed in nasal epithelial cells (at protein level) (PubMed:33432184, PubMed:32333915). Coexpressed with TMPRSS2 within some lung alveolar type 2 cells, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells (PubMed:32413319, PubMed:32327758, PubMed:32358202). Coexpressed with TMPRSS4 within mature enterocytes (PubMed:32404436).; [Isoform 2]: Expressed in nasal and bronchial epithelial cells (at protein level). |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Renin-angiotensin system |
| Research Areas | |
| Function | cofactor:Binds 1 chloride ion per subunit.,cofactor:Binds 1 zinc ion per subunit.,enzyme regulation:Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat.,Function:Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.,induction:Up-regulated in failing heart.,PTM:N-glycosylation on Asn-90 may limit SARS infectivity.,similarity:Belongs to the peptidase M2 family.,subunit:Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein.,tissue specificity:Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system., |
