Price range: $268.00 through $328.00
Background:
This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
| Size | 50μL/100μL |
| Target | ACSS1 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 75kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACSS1. AA range:611-660 |
| Specificity | ACSS1 Polyclonal Antibody detects endogenous levels of ACSS1 protein. |
| Gene Name | ACSS1 ACAS2L KIAA1846 |
| Protein Name | Acetyl-coenzyme A synthetase 2-like mitochondrial |
| Other Name | ACSS1; / ACAS2L; / KIAA1846; / Acetyl-coenzyme A synthetase 2-like; / mitochondrial; / Acetate--CoA ligase 2; / Acetyl-CoA synthetase 2; / AceCS2; / Acyl-CoA synthetase short-chain family member 1 |
| Species | Human |
| Gene ID | 84532 |
| Uniprot | Q9NUB1, |
| Species.1 | Mouse |
| Gene ID.1 | 68738 |
| Uniprot.1 | Q99NB1 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion matrix . |
| Tissue Expression | Amygdala,Brain,PCR rescued clones,Placenta,Spleen,Stomach,T |
| Signaling_pathway | |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Pyruvate metabolism; / >>Glyoxylate and dicarboxylate metabolism; / >>Propanoate metabolism; / >>Metabolic pathways; / >>Carbon metabolism |
| Function | Catalytic activity:ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.,Function:Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).,sequence Caution:Sequencing errors.,similarity:Belongs to the ATP-dependent AMP-binding enzyme family., |
