SKU: WP10933 Categories: ,

Actin α3 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP10933-100
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Product details

Background:

Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010],

Specifications

TargetActin α3
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)45kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human Actin-gamma2. AA range:1-50
Sequence
SpecificityActin α3 Polyclonal Antibody detects endogenous levels of Actin α3 protein.
Gene NameACTG2
Protein NameActin gamma-enteric smooth muscle
Other NameACTG2; / ACTA3; / ACTL3; / ACTSG; / Actin; / gamma-enteric smooth muscle; / Alpha-actin-3; / Gamma-2-actin; / Smooth muscle gamma-actin
SpeciesHuman
Gene ID-172
UniprotP63267,
Species.1Mouse
Gene ID-211468
Uniprot.1P63268,
Species.2Rat
Gene ID-325365
Uniprot.2P63269
Organism-4
Gene ID-4
SwissProt-4
BackgroundActins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010],
Cellular LocalizationCytoplasm, cytoskeleton.
Tissue ExpressionIn the intestine, abundantly expressed in smooth muscle cells of muscularis mucosa and muscularis propria. Also detected in intestinal vascular smooth muscle cells.
Signaling_pathwayOrganismal Systems >> Circulatory system >> Vascular smooth muscle contraction
Research Areas>>Vascular smooth muscle contraction
FunctionFunction:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the actin family.,subunit:Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.,
RRID
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