Actinin-α2 Monoclonal Antibody
Ucallm_WM0470-50
Price range: $268.00 through $328.00
Product details
Background:
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013],
Specifications
| Size | 50μL/100μL |
| Target | Actinin-α2 |
| Reactivity | Human, Rat, Bovine, Chicken, Dog, Pig, Zebrafish |
| Applications | WB, IF |
| MW(Calculated) | 104kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:2000; IF 1:100-1:500; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 6H8 |
| Immunogen | Purified recombinant human Actinin-α2 (C-terminus) protein fragments expressed in E.coli. |
| Specificity | Actinin-α2 Monoclonal Antibody detects endogenous levels of Actinin-α2 protein. |
| Gene Name | ACTN2/ACTN3 |
| Protein Name | Alpha-actinin-2 |
| Other Name | ACTN2; / Alpha-actinin-2; / Alpha-actinin skeletal muscle isoform 2; / F-actin cross-linking protein |
| Species | Human |
| Gene ID | 88 |
| Uniprot | P35609, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9JI91 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, myofibril, sarcomere, Z line . Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. |
| Tissue Expression | Expressed in both skeletal and cardiac muscle. |
| Signaling_pathway | |
| Research Areas | >>Arrhythmogenic right ventricular cardiomyopathy |
| Function | Disease:Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,Function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.,subunit:Homodimer; antiparallel. Also forms heterodimers with ACTN3. Interacts with ADAM12, MYOZ1, MYOZ2 and MYOZ3. Interacts via its C-terminal region with the LDB3 PDZ domain. Interacts with XIRP2.,tissue specificity:Expressed in both skeletal and cardiac muscle., |
Please login to post questions
Related products
