ADAR2 Polyclonal Antibody
Ucallm_WP10954-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | ADAR2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADARB1. AA range:481-530 |
| Specificity | ADAR2 Polyclonal Antibody detects endogenous levels of ADAR2 protein. |
| Gene Name | ADARB1 |
| Protein Name | Double-stranded RNA-specific editase 1 |
| Other Name | ADARB1; / ADAR2; / DRADA2; / RED1; / Double-stranded RNA-specific editase 1; / RNA-editing deaminase 1; / RNA-editing enzyme 1; / dsRNA adenosine deaminase |
| Species | Human |
| Gene ID | 104 |
| Uniprot | P78563, |
| Species.1 | Mouse |
| Gene ID.1 | 110532 |
| Uniprot.1 | Q91ZS8, |
| Species.2 | Rat |
| Gene ID.2 | 25367 |
| Uniprot.2 | P51400 |
| Cellular Localization | Nucleus . Nucleus, nucleolus . Shuttles between nucleoli and the nucleoplasm. .; [Isoform 1]: Nucleus . Nucleus, nucleolus .; [Isoform 2]: Nucleus . Nucleus, nucleolus . |
| Tissue Expression | Highly expressed in brain and heart and at lower levels in placenta. Fair expression in lung, liver and kidney. Detected in brain, heart, kidney, lung and liver (at protein level).; [Isoform 5]: Highly expressed in hippocampus and colon. Expressed in pediatric astrocytomas and the protein has a decreased RNA-editing activity. The decrease in RNA editing correlates with the grade of malignancy of the tumors, with the high grade tumors showing lower editing is seen. |
| Signaling_pathway | |
| Research Areas | |
| Function | Alternative products:Additional isoforms seem to exist,cofactor:Binds 1 inositol hexakisphosphate (IP6) per subunit.,Function:Editing of the messenger RNAs for glutamate receptor (GluR) subunits by site-selective adenosine deamination. Edits both the GluR-B Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently.,similarity:Contains 1 A to I editase domain.,similarity:Contains 2 DRBM (double-stranded RNA-binding) domains., |
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