Price range: $268.00 through $328.00
Background:
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011],
| Size | 50μL/100μL |
| Target | AFF4 |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 127kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 30-110 |
| Specificity | AFF4 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | AFF4 AF5Q31 MCEF HSPC092 |
| Protein Name | AF4/FMR2 family member 4 (ALL1-fused gene from chromosome 5q31 protein) (Protein AF-5q31) (Major CDK9 elongation factor-associated protein) |
| Other Name | |
| Species | Human |
| Gene ID | 27125 |
| Uniprot | Q9UHB7, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9ESC8 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Associates to transcriptionally active chromatin but not at snRNA genes. . |
| Tissue Expression | Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain. |
| Signaling_pathway | |
| Research Areas | >>Viral life cycle - HIV-1 |
| Function | developmental stage:Expressed in fetal heart, lung, brain and to a lower extent liver.,Disease:A chromosomal aberration involving AFF4 is found in acute lymphoblastic leukemia (ALL). Insertion ins(5; 11)(q31; q13q23) that forms a MLL-AFF4 fusion protein.,Function:May play a role in transcriptional regulation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the AF4 family.,subunit:Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb).,tissue specificity:Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain., |
