AFP alpha 1 Fetoprotein Monoclonal Antibody [17C5]
Ucallm_WM1016-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | AFP |
| Reactivity | Human |
| Applications | WB, IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 70kD |
| Host Species | Mouse |
| Isotype | IgG2a,kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000; IF 1:200; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 17C5 |
| Immunogen | Synthetic Peptide of AFP alpha 1 Fetoprotein |
| Specificity | The antibody detects endogenous AFP proteins. |
| Gene Name | AFP |
| Protein Name | Alpha-1-Fetoprotein(AFP) |
| Other Name | AFP; / HPAFP; / Alpha-fetoprotein; / Alpha-1-fetoprotein; / Alpha-fetoglobulin |
| Species | Human |
| Gene ID | 174 |
| Uniprot | P02771, |
| Species.1 | Mouse |
| Gene ID.1 | 11576 |
| Uniprot.1 | P02772, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P02773 |
| Cellular Localization | Secreted. |
| Tissue Expression | Plasma. Synthesized by the fetal liver and yolk sac. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Hippo signaling pathway |
| Research Areas | >>Hippo signaling pathway |
| Function | developmental stage:Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.,Function:Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.,online information:Alpha-fetoprotein entry,PTM:Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.,PTM:Sulfated.,similarity:Belongs to the ALB/AFP/VDB family.,similarity:Contains 3 albumin domains.,subunit:Dimeric and trimeric forms have been found in addition to the monomeric form.,tissue specificity:Plasma. Synthesized by the fetal liver and yolk sac., |
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