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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/Aldose Reductase Polyclonal Antibody
SKU: WP11014 Categories: ,

Aldose Reductase Polyclonal Antibody

Ucallm_WP11014-50

Price range: $268.00 through $328.00

SKU: WP11014-100
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Product details

Background:

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009],

Specifications

TargetAKR1B1
ReactivityHuman, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)36kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human AKR1B1. AA range:241-290
Sequence
SpecificityAldose Reductase Polyclonal Antibody detects endogenous levels of Aldose Reductase protein.
Gene NameAKR1B1
Protein NameAldose reductase
Other NameAKR1B1; / ALDR1; / Aldose reductase; / AR; / Aldehyde reductase; / Aldo-keto reductase family 1 member B1
SpeciesHuman
Gene ID-1231
UniprotP15121,
Species.1Mouse
Gene ID-211677
Uniprot.1P45376,
Species.2Rat
Gene ID-324192
Uniprot.2P07943
Organism-4
Gene ID-4
SwissProt-4
BackgroundThis gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009],
Cellular LocalizationCytoplasm.
Tissue ExpressionHighly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.
Signaling_pathway
Research Areas>>Pentose and glucuronate interconversions; / >>Fructose and mannose metabolism; / >>Galactose metabolism; / >>Glycerolipid metabolism; / >>Folate biosynthesis; / >>Metabolic pathways
FunctionCatalytic activity:Alditol + NAD(P)(+) = aldose + NAD(P)H.,Disease:In diabetes and galactosemia, increased AR activity leads to high levels of sorbitol and galactitol, respectively, in the cells of many tissues. Accumulation of sugar alcohols has been shown to cause osmotic cataracts in the lens. AR is also thought to play a key role in diabetic complications of three other target tissues, namely, nerve, kidney and retina.,enzyme regulation:Cys-299 may regulate the kinetic and inhibition properties of the enzyme, but does not participate in catalysis.,Function:Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.,similarity:Belongs to the aldo/keto reductase family.,subunit:Monomer.,tissue specificity:Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.,
RRID
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