Price range: $268.00 through $328.00
Background:
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | ALKBH2 |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 28kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 140-220 |
| Specificity | ALKB2 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | ALKBH2 ABH2 |
| Protein Name | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 (Alkylated DNA repair protein alkB homolog 2) (DNA oxidative demethylase ALKBH2) (Oxy DC1) |
| Other Name | |
| Species | Human |
| Gene ID | 121642 |
| Uniprot | Q6NS38, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q6P6J4 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Nucleus, nucleolus . Nucleus, nucleoplasm . Relocates to the replication foci during S-phase. . |
| Tissue Expression | Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. |
| Signaling_pathway | |
| Research Areas | |
| Function | cofactor:Binds 1 Fe(2+) ion per subunit.,enzyme regulation:Activated by ascorbate.,Function:Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.,similarity:Belongs to the alkB family.,subcellular location:Detected in replication foci during S-phase.,tissue specificity:Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder., |
