Name: AMBP Polyclonal Antibody
SKU: WP2427
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	AMBP
Reactivity	Human, Mouse, Rat
Applications	WB, ELISA
MW(Calculated)
MW(Observed)	38kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-2000; ELISA 1:5000-20000
Formulation	Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized peptide derived from part region of human protein
Specificity	AMBP Polyclonal Antibody detects endogenous levels of protein.
Gene Name	AMBP HCP ITIL
Protein Name	Protein AMBP [Cleaved into: Alpha-1-microglobulin (Protein HC) (Alpha-1 microglycoprotein) (Complex-forming glycoprotein heterogeneous in charge); Inter-alpha-trypsin inhibitor light chain (ITI-LC) (Bikunin) (EDC1) (HI-30) (Uronic-acid-rich protein); Trypstatin]
Other Name
Species	Human
Gene ID	259
Uniprot	P02760,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q07456,
Species.2	Rat
Gene ID.2
Uniprot.2	Q64240
Cellular Localization	[Alpha-1-microglobulin]: Secreted . Endoplasmic reticulum . Cytoplasm, cytosol . Cell membrane; Peripheral membrane protein . Nucleus membrane; Peripheral membrane protein . Mitochondrion inner membrane; Peripheral membrane protein . Secreted, extracellular space, extracellular matrix . The cellular uptake occurs via a non-endocytotic pathway and allows for localization to various membrane structures. A specific binding to plasma membrane suggests the presence of a cell receptor, yet to be identified. Directly binds collagen fibers type I. .
Tissue Expression	[Alpha-1-microglobulin]: Expressed by the liver and secreted in plasma. Occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid (PubMed:11877257). Expressed in epidermal keratinocytes, in dermis and epidermal-dermal junction (at protein level) (PubMed:22096585). Expressed in red blood cells (at protein level) (PubMed:32092412). Expressed in placenta (PubMed:21356557).; [Inter-alpha-trypsin inhibitor light chain]: Expressed in airway epithelium and submucosal gland (at protein level). Colocalizes with TNFAIP6 at the ciliary border. Present in bronchoalveolar lavage fluid (at protein level).
Signaling_pathway
Research Areas
Function	Function:Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.,Function:Trypstatin is a trypsin inhibitor.,miscellaneous:In vitro, the first twelve residues of the amino end of the inhibitor appear to have a reactive site capable of inhibiting the activity of a number of enzymes. Its in vivo function is not known.,PTM:3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.,PTM:Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.,PTM:Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.,PTM:The precursor is proteolytically processed into separately functioning proteins.,similarity:Contains 1 BPTI/Kunitz inhibitor domain.,similarity:Contains 2 BPTI/Kunitz inhibitor domains.,similarity:In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.,subunit:I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (H1, H2 or H3) and one light chain, bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of H1, H2 and bikunin, inter-alpha-like inhibitor (I-alpha-LI) of H2 and bikunin, and pre-alpha-inhibitor (P-alpha-I) of H3 and bikunin. Alpha-1-microglobulin occurs as a monomer and also in complexes with IgA and albumin. Alpha-1-microglobulin interacts with FN1. Trypstatin is a monomer and also occurs as a complex with tryptase in mast cells (By similarity). Alpha-1-microglobulin and bikunin interact (via SH3 domain) with HEV ORF3 protein.,tissue specificity:Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.,

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AMBP Polyclonal Antibody

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AMBP Polyclonal Antibody

Product details

Specifications

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