Price range: $268.00 through $328.00
Background:
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014],
| Size | 50μL/100μL |
| Target | Annexin I |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | WB |
| MW(Calculated) | |
| MW(Observed) | 38kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 12G2 |
| Immunogen | Purified recombinant human Annexin A1 beta protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of Annexin A1 and does not cross-react with related proteins. |
| Gene Name | anxa1 |
| Protein Name | |
| Other Name | Annexin 1; / Annexin A1; / Annexin I; / lipocortin I; / Annexin I; / Annexin-1; / AnnexinA1; / AnnexinI; / ANX 1; / ANX A1; / ANX1; / ANXA 1; / ANXA1; / ANXA1 protein; / ANXA1_HUMAN; / Calpactin 2; / Calpactin II; / Calpactin-2; / CalpactinII; / Chromobindin 9; / Chromobindin-9; / Chromobindin9; / HGNC:533; / Lipocortin 1; / Lipocortin I; / Lipocortin1; / LipocortinI; / LPC 1; / LPC1; / p35; / Phospholipase A2 inhibitory protein. |
| Species | Human |
| Gene ID | 301 |
| Uniprot | P04083, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P10107 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Cell projection, cilium . Cell membrane . Membrane; Peripheral membrane protein . Endosome membrane; Peripheral membrane protein . Basolateral cell membrane . Apical cell membrane . Lateral cell membrane . Secreted . Secreted, extracellular space . Cell membrane; Peripheral membrane protein; Extracellular side . Secreted, extracellular exosome . Cytoplasmic vesicle, secretory vesicle lumen . Cell projection, phagocytic cup . Early endosome . Cytoplasmic vesicle membrane; Peripheral membrane protein . Secreted, at least in part via exosomes and other secretory vesicles. Detected in exosomes and other extracellular vesicles (PubMed:25664854). Alternatively, the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in the protein translocation from the cytoplasm into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). Detected in gelatinase granules in resting neutrophils (PubMed:10772777). Secretion is increased in response to wounding and inflammation (PubMed:25664854). Secretion is increased upon T-cell activation (PubMed:17008549). Neutrophil adhesion to endothelial cells stimulates secretion via gelatinase granules, but foreign particle phagocytosis has no effect (PubMed:10772777). Colocalizes with actin fibers at phagocytic cups (By similarity). Displays calcium-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). . |
| Tissue Expression | Detected in resting neutrophils (PubMed:10772777). Detected in peripheral blood T-cells (PubMed:17008549). Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors (PubMed:25664854). Detected in placenta (at protein level) (PubMed:2532504). Detected in liver. |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:A pair of annexin repeats may form one binding site for calcium and phospholipid.,Function:Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.,PTM:Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.,similarity:Belongs to the annexin family.,similarity:Contains 1 annexin repeat.,similarity:Contains 2 annexin repeats.,similarity:Contains 4 annexin repeats.,subcellular location:Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.,subunit:Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF., |
