APLF Polyclonal Antibody
Ucallm_WP11072-50
Price range: $268.00 through $328.00
Product details
Background:
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007 [PubMed 17353262]).[supplied by OMIM, Mar 2008],
Specifications
| Size | 50μL/100μL |
| Target | APLF |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 57kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human APLF. AA range:82-131 |
| Specificity | APLF Polyclonal Antibody detects endogenous levels of APLF protein. |
| Gene Name | APLF |
| Protein Name | Aprataxin and PNK-like factor |
| Other Name | APLF; / C2orf13; / PALF; / XIP1; / Aprataxin and PNK-like factor; / Apurinic-apyrimidinic endonuclease APLF; / PNK and APTX-like FHA domain-containing protein; / XRCC1-interacting protein 1 |
| Species | Human |
| Gene ID | 200558 |
| Uniprot | Q8IW19, |
| Species.1 | Mouse |
| Gene ID.1 | 72103 |
| Uniprot.1 | Q9D842 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Chromosome . Cytoplasm, cytosol . Localizes to DNA damage sites (PubMed:18474613, PubMed:18172500, PubMed:21211721, PubMed:23689425). Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers (PubMed:18172500). . |
| Tissue Expression | Testis, |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:The FHA-like domain mediates interaction with XRCC1 and XRCC4.,Function:Involved in single-strand and double-strand DNA break repair.,PTM:Phosphorylated in an ATM-dependent manner upon double-strand DNA break.,similarity:Contains 1 FHA-like domain.,similarity:Contains 2 C2H2-type zinc fingers.,subcellular location:Colocalizes with XRCC1 at sites of DNA damage.,subunit:Interacts with XRCC1. May also interact with XRCC4 and XRCC5., |
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