Name: AR-β2 Polyclonal Antibody
SKU: WP11160
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],

Specifications

Target	Adrenergic Receptor β2
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, FC, ELISA
MW(Calculated)
MW(Observed)	47kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Adrenergic Receptor beta2. AA range:321-370
Sequence
Specificity	AR-β2 Polyclonal Antibody detects endogenous levels of AR-β2 protein.
Gene Name	ADRB2
Protein Name	Beta-2 adrenergic receptor
Other Name	ADRB2; / ADRB2R; / B2AR; / Beta-2 adrenergic receptor; / Beta-2 adrenoreceptor; / Beta-2 adrenoceptor
Species	Human
Gene ID-1	154
Uniprot	P07550,
Species.1	Mouse
Gene ID-2
Uniprot.1	P18762
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],
Cellular Localization	Cell membrane; Multi-pass membrane protein . Early endosome . Golgi apparatus . Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325). Activated receptors are also detected within the Golgi apparatus (PubMed:27481942). .
Tissue Expression	Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid,
Signaling_pathway	Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway
Research Areas	>>Calcium signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Adrenergic signaling in cardiomyocytes; / >>Regulation of lipolysis in adipocytes; / >>Renin secretion; / >>Salivary secretion; / >>Chemical carcinogenesis - receptor activation
Function	Disease:Polymorphic forms of ADRB2 could impart some form of nocturnal asthma.,Function:Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.,PTM:Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.,PTM:Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Binds SLC9A3R1 and GPRASP1.,
RRID

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AR-β2 Polyclonal Antibody

Product details

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AR-β2 Polyclonal Antibody

Product details

Specifications

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