SKU: WP11120 Categories: ,

ARHGAP22 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11120-100
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Product details

Background:

This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],

Specifications

Size50μL/100μL
TargetARHGAP22
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)77kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human RHG22. AA range:565-614
SpecificityARHGAP22 Polyclonal Antibody detects endogenous levels of ARHGAP22 protein.
Gene NameARHGAP22
Protein NameRho GTPase-activating protein 22
Other NameARHGAP22; / RHOGAP2; / Rho GTPase-activating protein 22; / Rho-type GTPase-activating protein 22
SpeciesHuman
Gene ID58504
UniprotQ7Z5H3,
Species.1Mouse
Gene ID.1
Uniprot.1Q8BL80
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm . Nucleus . Mainly cytoplasmic. Some fraction is nuclear (By similarity). .
Tissue ExpressionBrain,Erythroleukemia,Lung,
Signaling_pathway
Research Areas
FunctionFunction:Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.,similarity:Contains 1 PH domain.,similarity:Contains 1 Rho-GAP domain.,subcellular location:Mainly cytoplasmic. Some fraction is nuclear.,subunit:Interacts with VEZF1.,
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