Price range: $268.00 through $328.00
Background:
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Asf1a |
| Reactivity | Human, Mouse |
| Applications | WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 23kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ASF1A. AA range:121-170 |
| Specificity | Asf1a Polyclonal Antibody detects endogenous levels of Asf1a protein. |
| Gene Name | ASF1A |
| Protein Name | Histone chaperone ASF1A |
| Other Name | ASF1A; / CGI-98; / HSPC146; / Histone chaperone ASF1A; / Anti-silencing function protein 1 homolog A; / hAsf1; / hAsf1a; / CCG1-interacting factor A; / CIA; / hCIA |
| Species | Human |
| Gene ID | 25842 |
| Uniprot | Q9Y294, |
| Species.1 | Mouse |
| Gene ID.1 | 66403 |
| Uniprot.1 | Q9CQE6 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Ubiquitously expressed. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.,PTM:Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.,similarity:Belongs to the ASF1 family.,subunit:Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1.,tissue specificity:Ubiquitously expressed., |
