Name: U-Blot® ATF-4 Rabbit mAb
SKU: WR4148
Price: 268 USD
Availability: InStock

Product details

Background:activating transcription factor 4(ATF4) Homo sapiens This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provid

Specifications

Size	50μL/100μL
Target	CREB-2
Reactivity	Human, Mouse, Rat, Pig
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	38kD
MW(Observed)	50kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:100-1:200; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0294R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	ATF4
Protein Name	Cyclic AMP-dependent transcription factor ATF-4
Other Name	ATF4; / CREB2; / TXREB; / Cyclic AMP-dependent transcription factor ATF-4; / cAMP-dependent transcription factor ATF-4; / Activating transcription factor 4; / Cyclic AMP-responsive element-binding protein 2; / CREB-2; / cAMP-responsive element-binding prot
Species	Human
Gene ID	468
Uniprot	P18848,
Species.1	Mouse
Gene ID.1	11911
Uniprot.1	Q06507
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus
Tissue Expression	Bladder,Colon,Fibroblast,Leukemic T-cell,Lung,Ovary,Placenta,
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>MAPK signaling pathway; / >>cGMP-PKG signaling pathway; / >>Mitophagy - animal; / >>Protein processing in endoplasmic reticulum; / >>PI3K-Akt signaling pathway; / >>Apoptosis; / >>Longevity regulating pathway; / >>Adrenergic signaling in cardiomyocytes; / >>TNF signaling pathway; / >>Long-term potentiation; / >>Neurotrophin signaling pathway; / >>Cholinergic synapse; / >>Dopaminergic synapse; / >>Insulin secretion; / >>GnRH signaling pathway; / >>Estrogen signaling pathway; / >>Thyroid hormone synthesis; / >>Glucagon signaling pathway; / >>Aldosterone synthesis and secretion; / >>Relaxin signaling pathway; / >>Cortisol synthesis and secretion; / >>Parathyroid hormone synthesis, secretion and action; / >>Non-alcoholic fatty liver disease; / >>Cushing syndrome; / >>Growth hormone synthesis, secretion and action; / >>Alzheimer disease; / >>Parkinson disease; / >>Amyotrophic lateral sclerosis; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Cocaine addiction; / >>Amphetamine addiction; / >>Alcoholism; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Viral carcinogenesis; / >>Chemical carcinogenesis - receptor activation; / >>Prostate cancer; / >>Lipid and atherosclerosis
Function	Function:Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subcellular location:Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.,subunit:Interacts with the C-terminal region of GABBR1 via the leucine zipper of its C-terminal bZIP domain. Interacts with the C-terminal region of GABBR2 (By similarity). Binds DNA as a homo-or heterodimer. Interacts with the N-terminal region of CEP290.,