SKU: WP10807 Categories: ,

B-Myb (Phospho Thr494/497) Rabbit pAb

Price range: $288.00 through $368.00

SKU: WP10807-100
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Product details

Background:

MYB proto-oncogene like 2(MYBL2) Homo sapiens The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013],

Specifications

Size50μL/100μL
TargetB-Myb Phospho Thr494
ReactivityHuman, Mouse
ApplicationsIHC, WB
MW(Calculated)
MW(Observed)80kD
Host SpeciesRabbit
Isotype
Conjugate/Modificationphosphate
Recommended Dilution RatioWB 1:500-2000; IHC 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from human B-Myb (Phospho Thr494/497)
SpecificityThis antibody detects endogenous B-Myb when phosphorylated at thr494(human),thr497(mosue)
Gene NameMYBL2 BMYB
Protein NameMyb-related protein B (B-Myb) (Myb-like protein 2)
Other NameMyb-related protein B; / B-Myb; / Myb-like protein 2
SpeciesHuman
Gene ID4605
UniprotP10244,
Species.1Mouse
Gene ID.117865
Uniprot.1P48972
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus.
Tissue Expression
Signaling_pathwayCellular Processes >> Cell growth and death >> Cellular senescence
Research Areas>>Cellular senescence
FunctionFunction:Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.,PTM:Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.,similarity:Contains 3 HTH myb-type DNA-binding domains.,subunit:Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL22.,
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