Bad Polyclonal Antibody
Ucallm_WP11231-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Bad |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 18kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BAD. AA range:78-127 |
| Specificity | Bad Polyclonal Antibody detects endogenous levels of Bad protein. |
| Gene Name | BAD |
| Protein Name | Bcl2 antagonist of cell death |
| Other Name | BAD; / BBC6; / BCL2L8; / Bcl2 antagonist of cell death; / BAD; / Bcl-2-binding component 6; / Bcl-2-like protein 8; / Bcl2-L-8; / Bcl-XL/Bcl-2-associated death promoter |
| Species | Human |
| Gene ID | 572 |
| Uniprot | Q92934, |
| Species.1 | Mouse |
| Gene ID.1 | 12015 |
| Uniprot.1 | Q61337, |
| Species.2 | Rat |
| Gene ID.2 | 64639 |
| Uniprot.2 | O35147 |
| Cellular Localization | Mitochondrion outer membrane. Cytoplasm . Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm. . |
| Tissue Expression | Expressed in a wide variety of tissues. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>Platinum drug resistance; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>Autophagy - animal; / >>PI3K-Akt signaling pathway; / >>Apoptosis; / >>VEGF signaling pathway; / >>Focal adhesion; / >>Neurotrophin signaling pathway; / >>Insulin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Alzheimer disease; / >>Amyotrophic lateral sclerosis; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Toxoplasmosis; / >>Tuberculosis; / >>Hepatitis C; / >>Hepatitis B; / >>Measles; / >>Human papillomavirus infection; / >>Herpes simplex virus 1 infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Pancreatic cancer; / >>Endometrial cancer; / >>Prostate cancer; / >>Melanoma; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Non-small cell lung cancer; / >>Hepatocellular carcinoma; / >>Lipid and atherosclerosis |
| Function | Domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,Function:Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.,online information:Bcl 2-associated death promoter entry,PTM:Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.,similarity:Belongs to the Bcl-2 family.,subcellular location:Upon phosphorylation, locates to the cytoplasm.,subunit:Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins.,tissue specificity:Expressed in a wide variety of tissues., |
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